Cell Surface Protein Isolation Kit

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SKU:
K295-10
€415
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Description

Cell Surface Protein Isolation Kitis available at Gentaur for Next week Delivery.

Simple and efficient method for isolation of cell surface proteins.

Description:

Cell surface proteins play a major role in signal transduction, cell adhesion, transport and serve as diagnostic and pharmacological targets. BioVision’s Cell Surface Protein Isolation Kit provides a simple and efficient method for the isolation of cell surface proteins. In this method, cells are first labeled with Sulfo-NHS-SS-Biotin, an amine-reactive, thiol-cleavable, biotinylation reagent. Cells are subsequently lysed and the labeled cell surface proteins are isolated using Streptavidin beads. The bound proteins are then released from beads by incubating with DTT solution. Biotinylation reagent is cell-membrane-impermeable with an extended spacer arm to reduce steric hindrances associated with streptavidin binding. This convenient kit provides all the required components for optimal labeling and isolation of the cell surface proteins.

Applications: Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies

Sample Typ:e Adherent and suspension cells

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Features and Benefits:

• Simple and efficient method for the isolation of cell surface proteins • Includes all required components for optimal labeling and isolation of the cell surface proteins

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Additional Information

Size:
10 reactions
Storage Conditions:
4°C4°C
Shipping Conditions:
gel pack
Shelf life:
12 months
Detection Method:
Western Blotting
Category:
Protein Extraction & Purification, cell surface protein isolation
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