CaspSCREEN™ Flow Cytometric Apoptosis Detection Kit

CaspSCREEN™ Flow Cytometric Apoptosis Detection Kitis available at Gentaur for Next week Delivery.

Measures Activated Caspases in intact cells by FACS.

Description:

Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D₂R), a reported substrate for members of caspase family proteases. The caspase substrate D₂R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.

Applications:

Sample Typ:e Live cells

Alternate Name:

Features and Benefits:

• Simple one-step procedure; takes only 20-30 minutes • Fast and convenient • D₂R is more cell-permeable than other fluorometric caspase substrates