Caspase-9 Colorimetric Assay Kit

Caspase-9 Colorimetric Assay Kitis available at Gentaur for Next week Delivery.

Convenient, Highly-Sensitive Assay.

Description:

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

Applications:

Sample Typ:e Cell and tissue lysates

Alternate Name:

Features and Benefits:

• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity.