Caspase-3 Fluorometric Assay Kit

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SKU:
K105-400
  • Caspase-3 Fluorometric Assay Kit
  • Caspase-3 Fluorometric Assay Kit
  • Caspase-3 Fluorometric Assay Kit
  • Caspase-3 Fluorometric Assay Kit
£625.62
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Description

Caspase-3 Fluorometric Assay Kitis available at Gentaur for Next week Delivery.

Convenient, Highly-Sensitive Assay.

Description:

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.

Applications:

Sample Typ:e Cell and tissue lysates

Alternate Name:

Features and Benefits:

• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity

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Additional Information

Size:
400 assays
Storage Conditions:
4°C-20°C
Shipping Conditions:
gel pack
Shelf life:
12 months
Category:
Apoptosis
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