Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit

Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kitis available at Gentaur for Next week Delivery.

Highly Sensitive Assay.

Description:

α-Ketoglutarate (α-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing α-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. α-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. BioVision's α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples. In the assay, α-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The α-Ketoglutarate Assay Kit is useful for detecting α-ketoglutarate in the range of 0.01 to 10 nmoles.

Applications:

Sample Typ:e Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids

Alternate Name:

Features and Benefits:

• Simple procedure; takes ~ 30 minutes to complete the assay • Fast and convenient • Highly sensitive assays for measuring alpha-Ketaglutarate levels in biological samples