Phospho-Chk2 (Ser516) Antibody

Phospho-Chk2 (Ser516) Antibodyis available at Gentaur for Next week Delivery.

Description: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression.

Immunogen Sequence:Synthesized peptide derived from human Chk2 around the phosphorylation site of S516

Target Alternative Name:Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2, CHEK2

Species Reactivities:Human, Monkey

Tag Line:Polyclonal antibody to detect endogenous levels of Chk2 (pS516) in human and monkey samples

Formulation:In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Purification: Affinity-chromatography using epitope-specific immunogen

Application:WB, ELISA

Positive Control:WB: COS 7 cell lysate

Application And Usages:WB 1:500-1:2000; ELISA 1:10000

Handling:The antibody solution should be gently mixed before use