Phospho-Chk1 (Ser280) Antibody

Phospho-Chk1 (Ser280) Antibodyis available at Gentaur for Next week Delivery.

Description: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C.

Immunogen Sequence:Synthesized peptide derived from human Chk1 around the phosphorylation site of S280

Target Alternative Name:Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1

Species Reactivities:Human

Tag Line:Polyclonal antibody to detect endogenous levels of Chk1 (pS280) in human samples

Formulation:In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Purification: Affinity-chromatography using epitope-specific immunogen

Application:WB, ELISA

Positive Control:WB: 293T cell lysate

Application And Usages:WB 1:500-1:2000; ELISA 1:20000

Handling:The antibody solution should be gently mixed before use