DNA-PK Antibody

DNA-PK Antibodyis available at Gentaur for Next week Delivery.

Description: DNA-dependent protein kinase (DNA-PK) is made up of 3 subunits, 2 Ku proteins and a large catalytic subunit (DNA-PKcs). DNA-PKcs falls into the phosphatidyl-inositol 3-kinase superfamily althoμgh it does have serine/threonine protein kinase activity. This subunit migrates on SDS-PAGE gels as an intact and smaller processed form. In vitro, DNA-PK phosphorylates several transcription factors and other DNA-binding proteins and is believed to play a role in DNA damage recognition, repair and transcription. There is evidence to sμggest that DNA-PKcs may be a critical target for proteolysis by a caspase in apoptosis.

Immunogen Sequence:Synthetic peptide corresponding to residues surrounding amino acid 4088 of human DNA-PK

Target Alternative Name:PRKDC, XRCC7 , p460, DNPK1, HYRC, HYRC1 , DNAPK ,p350

Species Reactivities:Human, mouse, rat, hamster

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Formulation:100 µg (0.5 mg/ml) immunoaffinity purified rabbit anti-DNA-PK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application:Western blot

Positive Control:/

Application And Usages:Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects a >350,000 kDa and a 180,000 kDa protein (believed to be a processed form of the catalytic subunit), corresponding to the expected molecular weight of DNA-PKcs, in samples from human, mouse, rat and hamster origins.

Handling:The antibody solution should be gently mixed before use.