Description
USP8 Antibodyis available at Gentaur for Next week Delivery.
Description: Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action. Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. The deubiquitinating enzyme ubiquitin-specific protease 8 (USP8/UBPy) is a cysteine protease belonging to the USP/UBP subfamily. Research studies have shown that USP8 is an essential growth-regulated enzyme indispensible for cell proliferation and survival. Indeed, conditional knock-out of murine USP8 was shown to promote a dramatic loss in expression of receptor tyrosine kinases, including EGFR, ErbB3, and c-Met. In agreement with these findings, USP8 inactivation leads to enhanced ubiquitination of ligand-activated EGFR. Furthermore, phosphorylation of USP8 at Ser680 results in its binding of 14-3-3, catalytic inactivation, and reduced EGFR deubiquitination. It appears as though USP8, in conjunction with components of the ESCRT-0 complex, plays an integral role in the early endosomal sorting machinery that functions to protect EGFR from lysosomal degradation.
Immunogen Sequence:Synthetic peptide corresponding to an internal sequence of human USP 8 (BV-L43)
Target Alternative Name:Deubiquitinating enzyme 8, hUBPy, HumORF8, KIAA0055, mgC129718, Ubiquitin carboxyl-terminal hydrolase 8, Ubiquitin-specific processing protease 8, Ubiquitin thioesterase 8, UBP, Ubiquitin Specific Protease 8.
Species Reactivities:Human
Tag Line:Polyclonal Antibody to detect USP8 in human samples
Formulation:In phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.
Purification: Affinity Purified Antibody
Application:Western blot
Positive Control:Western blot: Jurkat Cell lysate
Application And Usages:Western blot: 1-4 µg/ml.
Handling:The antibody solution should be gently mixed before use.
Additional Information
Size: |
30 μg |
Antibody Target: |
USP8 |
Category: |
USP8 |
Lot Number: |
9D186658 |
Host: |
Rabbit |
Antibody Type: |
Polyclonal |
Isotype: |
Rabbit IgG |
Accession Number: |
P40818 |
Gene Id: |
9101 |
Concentration: |
0.5 mg/ml |
Appearance: |
Colorless liquid |
Form: |
Liquid |
Storage Condition: |
-20 °C |
Shipping Condition: |
gel pack |
Shelf Life: |
12 months |
Country of Animal Origin: |
USA |
Country of Manufacture: |
USA |
Usage: |
For Research Use Only! Not to be used in humans. |
Western Blot Verified: |
Verified |
Immunocytochemistry Verified: |
Not Verified |
Immunofluorescence Verified: |
Not Verified |
Immunoprecipitation Verified: |
Not Verified |
ELISA Verified: |
Not Verified |
ChIP Verified: |
Not Verified |
Flow Cytometry Verified: |
Not Verified |