Thioredoxin 2 Antibody (4C5)

Thioredoxin 2 Antibody (4C5)is available at Gentaur for Next week Delivery.

Description: The mammalian thioredoxin reductases (TrxRs) are a family of seleno-cysteine containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate seleno-cysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55 – 58 kDa in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. Trx 2 Has an anti-apoptotic function and plays an important role in the regulation of mitochondrial membrane potential. Could be involved in the resistance to anti-tumor agents. Possesses a dithiol-reducing activity.

Immunogen Sequence:Recombinant human protein purified from E.coli

Target Alternative Name:MTRX, TRX2, MT-TRX.

Species Reactivities:Human, Mouse, Rat

Tag Line:Monoclonal Antibody to detect thioredoxin II in human, mouse and rat samples.

Formulation:100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol

Purification: Ammonium sulphate precipitation purified antibody

Application:Western blot, IP

Positive Control:IP analysis: 293T cell lysates

Application And Usages:Western blot: 1 µg/ml, IP: 2 µg.

Handling:The antibody solution should be gently mixed before use.