ROCK1 Antibody (Clone 12M03)

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SKU:
3545-100
$292
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Description

ROCK1 Antibody (Clone 12M03)is available at Gentaur for Next week Delivery.

Description: ROCK-1 (Rho-associated coiled coil-containing protein kinase 1) has been found to be a new caspase-3 substrate. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain. During apoptosis, ROCK-1 is cleaved by caspase-3 at the conserved DETD1113/G sequence and its C-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3 mediated cleavage and activation of ROCK-1 induces phosphorylation of MLC and membrane blebbing, the early events in the execution phase of apoptosis.

Immunogen Sequence:Synthetic peptide corresponding to the caspase-3 cleavage site

Target Alternative Name:ROCK1 , P160ROCK , mgC131603 , mgC43611 , p160ROCK , EC 2.7.11.1

Species Reactivities:Human

Tag Line:/

Formulation:100 µg (0.5 mg/ml) Protein G purified mouse anti-human ROCK1 monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Application:Western blot

Positive Control:/

Application And Usages:Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate treated with camptothecin (2 µM for 6-8 hours) can be used as a positive control. The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.

Handling:The antibody solution should be gently mixed before use.

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Additional Information

Size:
100 µg
Antibody Target:
ROCK-1 (cleaved)
Category:
ROCK
Lot Number:
50845
Host:
Mouse
Antibody Type:
Monoclonal
Isotype:
Mouse IgG
Accession Number:
Q13464
Gene Id:
6093
Concentration:
0.5 mg/ml
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20 °C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
USA
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans.
Western Blot Verified:
Verified
Immunocytochemistry Verified:
Not Verified
Immunofluorescence Verified:
Not Verified
Immunoprecipitation Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Flow Cytometry Verified:
Not Verified
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