Rabex-5 Antibody

Rabex-5 Antibodyis available at Gentaur for Next week Delivery.

Description: Rabex-5, also called RabGEF1 and RAP1, was identified as a guanine nucleotide exchange factor (GEF) for Rab5, a member of the Ras superfamily of small Rab GTPases (1). Rabex-5 generates the GTP-bound active form of Rab5 and forms a tight association with its effector protein Rabaptin-5. This complex localizes to endosomal membranes where it functions as a key regulator of vesicular trafficking during early endocytosis. Rabex-5 is also monoubiquitinated and has ubiquitin ligase activity that regulates its recruitment to early endosomes. The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis. Through its control of endosomal trafficking and endocytosis, Rabex-5 has been shown to negatively regulate NGF-mediated neurite outgrowth as well as FcεRI-dependent mast cell activation.

Immunogen Sequence:Synthetic peptide at C-terminal (BV-M11)

Target Alternative Name:Rab5 GDP/GTP exchange factor

Species Reactivities:Human, mouse, rat

Tag Line:Polyclonal antibody to detect Rabex-5 in human, mouse and rat samples

Formulation:100 µg (0.5 mg/ml) of antibody in PBS, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol, pH 7.2

Purification: Affinity purified

Application:Western blot

Positive Control:Rat Kidney lysate, 3T3 cell lysate

Application And Usages:Western blot: 1:200

Handling:The antibody solution should be gently mixed before use.