Phospho-Raf Antibody

Phospho-Raf Antibodyis available at Gentaur for Next week Delivery.

Description: c-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity, and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase.

Immunogen Sequence:Synthetic phospho-peptide surrounding amino acid Ser259 of human c-Raf

Target Alternative Name:RAF1 , cRaf , Raf-1 , c-Raf , RAF , CRAF , C-RAF, RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Species Reactivities:Human, mouse, rat

Tag Line:/

Formulation:100 µg (0.5 mg/ml) affinity purified rabbit anti-Phospho-Raf (Ser259) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Application:Western blot, Immunohistochemistry

Positive Control:/

Application And Usages:Western blotting (0.5-4 µg/ml) and Immunohistochemistry (20-40µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~74 kDa c-Raf when phosphorylated at Ser259.

Handling:The antibody solution should be gently mixed before use.