Phospho-JNK/SAPK Antibody

Phospho-JNK/SAPK Antibodyis available at Gentaur for Next week Delivery.

Description: The JNK pathway is activated by largely distinct stimuli including inflammatory cytokines (such as TNF-α and IL-1), UV light, inhibitors of protein synthesis and osmotic stress. Activated MEKK1 phosphorylates SEK1 (also known as MKK4), which in turn activates SAPK (also known as JNK). JNK binds tightly to the N-terminal region of c-Jun and ATF-2, and phosphorylates c-Jun at Ser63 and Ser73 and ATF-2 at Thr69 and Thr71.

Immunogen Sequence:Synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human SAPK/JNK

Target Alternative Name:c-Jun kinase, JNK , JNK 1 , JAK1A , MAPK8 , MAPK 8 , SAPK 1 , SAPK1 , Kinase JNK1 , Stress activated protein kinase

Species Reactivities:Human, mouse, rat

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Formulation:100 µg (0.5 mg/ml) affinity purified rabbit anti-phospho-JNK/SAPK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

Purification: Affinity purified

Application:Western blot

Positive Control:/

Application And Usages:Western blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), immunocytochemistry (10-20 µg/ml), and Immunohistochemistry (20-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the p46 and p54 JNK/SAPK dually phosphorylated at Thr183 and Tyr185.

Handling:The antibody solution should be gently mixed before use.