Phospho-JKK1/SEK1/MKK4 Antibody

Phospho-JKK1/SEK1/MKK4 Antibodyis available at Gentaur for Next week Delivery.

Description: SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs throμgh phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.

Immunogen Sequence:Synthetic peptide surrounding Ser80 of human SEK1/MKK4

Target Alternative Name:MAP2K4, SERK1, MAPKKA, JNKK1, PRKMK4, MEK4

Species Reactivities:Human, mouse, rat

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Formulation:100 µg (0.5 mg/ml) peptide affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.

Purification: Affinity purified

Application:Western blot

Positive Control:/

Application And Usages:Western blotting (1-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes phosphorylated SEK1/MKK4/JKK1 of human, mouse, and rat origins.

Handling:The antibody solution should be gently mixed before use.