Phospho-C/EBP β (Thr235) Antibody

Phospho-C/EBP β (Thr235) Antibodyis available at Gentaur for Next week Delivery.

Description: Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation.

Immunogen Sequence:Synthesized peptide derived from human C/EBP β around the phosphorylation site of T235. AA: 180-260

Target Alternative Name:CCAAT/enhancer-binding protein beta, C/EBP beta, LAP, Liver activator protein, Liver-enriched inhibitory protein, LIP, Nuclear factor NF-IL6, Transcription factor 5, TCF-5

Species Reactivities:Human, Mouse, Rat

Tag Line:Polyclonal antibody to detect endogenous levels of C/EBP β (pT235) in human, mouse and rat samples

Formulation:In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Purification: Affinity-chromatography using epitope-specific immunogen

Application:WB, ELISA, IHC, IF

Positive Control:WB: HepG2 cell lysate

Application And Usages:WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000

Handling:The antibody solution should be gently mixed before use