Phospho-AKT1 (Tyr474) polyconal Antibody

Phospho-AKT1 (Tyr474) polyconal Antibodyis available at Gentaur for Next week Delivery.

Description: The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Immunogen Sequence:Synthetic peptide

Target Alternative Name:PKB, c-Akt, RAC

Species Reactivities:Human

Tag Line:Polyclonal antibody to detect AKT1 (Phospho-Tyr474) in human samples.

Formulation:100 ug (1 mg/ml) of antibody in 0.01M Tris-HCl, pH 8.0, 0.15M NaCl, and 0.02% sodium azide.

Purification: Affinity purified rabbit IgG using protein G

Application:WB, Immunoblot, ELISA

Positive Control:N/A

Application And Usages:Western blot, Immunoblot: 0.5-2 μg/ml, ELISA

Handling:The antibody solution should be gently mixed before use.