Phospho-Akt1 (Ser246) Antibody

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SKU:
A1245-100
€285
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Description

Phospho-Akt1 (Ser246) Antibodyis available at Gentaur for Next week Delivery.

Description: The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Immunogen Sequence:Synthesized peptide derived from human ASK 1 around the phosphorylation site of S83, (20-100aa)

Target Alternative Name:AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Species Reactivities:Human, mouse , rat

Tag Line:Polyclonal antibody to detect endogenous levels of Akt1 protein phosphorylated at S246 in human, mouse and rat.

Formulation:In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Purification: Affinity-chromatography using epitope-specific immunogen

Application:WB, IHC, IF, ELISA

Positive Control:WB: 3T3, K562, HeLa cell lysate

Application And Usages:WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000

Handling:The antibody solution should be gently mixed before use

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Additional Information

Size:
100 µl
Antibody Target:
Akt1
Category:
Signalling
Lot Number:
N/A
Host:
Rabbit
Antibody Type:
Polyclonal
Isotype:
Rabbit IgG
Accession Number:
P31749
Gene Id:
207
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20°C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
USA
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans
Western Blot Verified:
Verified
Immunocytochemistry Verified:
Not Verified
Immunofluorescence Verified:
Not Verified
Immunoprecipitation Verified:
Not Verified
FACS Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Dot Blot Verified:
Not Verified
Flow Cytometry Verified:
Not Verified
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