Peroxiredoxin IV Antibody (3A1)

Peroxiredoxin IV Antibody (3A1)is available at Gentaur for Next week Delivery.

Description: Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx IV is probably involved in redox regulation of the cell. Regulates the activation of NFkappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.

Immunogen Sequence:Recombinant human fragment protein (without secretion leader sequence) purified from E.coli.

Target Alternative Name:Peroxiredoxin 4, PRX-4, AOE37-2, PRDX4.

Species Reactivities:Human

Tag Line:Monoclonal Antibody to detect peroxiredoxin IV in human samples.

Formulation:100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol

Purification: Ammonium sulphate precipitation purified antibody

Application:Western blot,IP,ICC

Positive Control:IP analysis : HeLa cell lysate. IHC staining : HeLa cells

Application And Usages:Western blot: 2-4 µl/ml, IP: 1-2 µl, ICC: 20 µl/ml.

Handling:The antibody solution should be gently mixed before use.