PARP Antibody

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SKU:
3002-100
€245
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Description

PARP Antibodyis available at Gentaur for Next week Delivery.

Description: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.

Immunogen Sequence:Synthetic peptide corresponding to caspase cleavage site in human PARP

Target Alternative Name:PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1

Species Reactivities:Human

Tag Line:/

Formulation:In phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Purification: Affinity purified

Application:Western blot

Positive Control:/

Application And Usages:Western blot analysis (1-4 µg/ml). The polyclonal anti-PARP antibody recognizes the full length (116 kDa) and the cleaved fragment (85 kDa) of human PARP. However, the optimal concentrations should be determined individually.

Handling:The antibody solution should be gently mixed before use.

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Additional Information

Size:
100 µg
Antibody Target:
PARP
Category:
PARP
Lot Number:
50802
Host:
Rabbit
Antibody Type:
Polyclonal
Isotype:
Rabbit IgG
Accession Number:
NP_001609
Gene Id:
142
Concentration:
0.2 mg/ml
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20 °C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
China
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans.
Western Blot Verified:
Verified
Immunocytochemistry Verified:
Not Verified
Immunofluorescence Verified:
Not Verified
Immunoprecipitation Verified:
Not Verified
FACS Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Dot Blot Verified:
Not Verified
Flow Cytometry Verified:
Not Verified
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