PARP Antibody (Clone 10H)

PARP Antibody (Clone 10H) is available at Gentaur for Next week Delivery.

Description: PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.

Immunogen Sequence: /

Target Alternative Name: PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1

Species Reactivities: All species

Tag Line: /

Formulation: 1 mg/ml of IgG purified from mouse ascites by protein A chromatography. Prepared in 20 mM Tris, pH 7.4, 150 mM NaCl, 1% BSA and 0.02% sodium azide.

Purification: Chromatography

Application: Western blot, Immunoprecipitation, Immunocytochemistry & ELISA

Positive Control: Tested by ICC in rat liver tissues

Application And Usages: Immunocytochemistry : 5-20 μg/ml; Western Blot (10 μg/ml using colorimetric methods; <2 μg/ml for ECL); Immunoprecipitation and ELISA

Handling: The antibody solution should be gently mixed before use.