NFkB p65 Antibody

NFkB p65 Antibodyis available at Gentaur for Next week Delivery.

Description: NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.

Immunogen Sequence:Synthetic peptide surrounding amino acid 274 of mouse NFkB p65

Target Alternative Name:RELA, NFKB3, mgC131774

Species Reactivities:Human, mouse, rat

Tag Line:/

Formulation:30 µg (0.2 mg/ml) affinity purified rabbit anti-NFkB polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 25% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application:Western blot, Immunohistochemistry

Positive Control:/

Application And Usages:Western blotting (0.5-4 µg/ml), immunocytochemistry (10-20µg/ml). However, the optimal conditions should be determined individually. The antibody detects primarily NFkB p65 in samples of human, mouse, and rat origins.

Handling:The antibody solution should be gently mixed before use.