NFkB p50 Polyclonal Antibody

NFkB p50 Polyclonal Antibodyis available at Gentaur for Next week Delivery.

Description: Nuclear factor kappa B (NFkB) was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.

Immunogen Sequence:Synthetic peptide mapping to the N-terminus of human NFkB p50.

Target Alternative Name:DNA binding factor KBF1 EBP1

Species Reactivities:Human, Mouse, Rat

Tag Line:Polyclonal Antibody to detect NFKB-p50 in Human, Mouse, Rat samples

Formulation:100 µg (0.5 mg/ml) rabbit anti-NFkB p50 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified rabbit IgG

Application:Western blot

Positive Control:N/A

Application And Usages:Western blot: 1-4 µg

Handling:The antibody solution should be gently mixed before use.