LC3 (APG8A) (NT) Antibody

LC3 (APG8A) (NT) Antibodyis available at Gentaur for Next week Delivery.

Description: Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.

Immunogen Sequence:This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).

Target Alternative Name:MAP1LC3A; Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain 3 alpha

Species Reactivities:Human, Mouse, Rat.

Tag Line:Polyclonal antibody to detect LC3 in human, mouse and rat samples

Formulation:Supplied in PBS with 0.09% (W/V) sodium azide.

Purification: Ammonium sulphate precipitated and dialyzed Antibody

Application:Western blot, Immunohistochemistry, IF

Positive Control:WB: rat brain lysate, IHC: human brain tissue, IF: U251 cells

Application And Usages:WB: 1:1000, IF: 1:200, IHC: 1:50-100.

Handling:The antibody solution should be gently mixed before use.