Description
JMJD2A Antibodyis available at Gentaur for Next week Delivery.
Description: Jumonji Domain containing 2A (JMJD2A) is a lysine specific demethylase with emerging roles in histone modification or epigenetic remodeling. This JMJD2A polyclonal antibody was raised against an N-terminal recombinant fragment of JMJD2A. This fragment includes amino acids 1-350 including the JMJN and JMJC domains but not the two LAP/PHD zinc finger or Tudor domains of the 1,064 amino acid protein. JMJD2A is detected at 135 kDa by western blotting using a DLD1 cell lysate as a positive control. Also, the expression of JMJD2A varies by tissue, and northern blotting sμggests skeletal muscle as a negative control and lung as a positive control tissue.
Immunogen Sequence:Human recombinant JMJD2A aa 1-350
Target Alternative Name:Jumonji Domain Containing 2A; KDM4A; Lysine-Specific Demethylase 4A
Species Reactivities:Human JMJD2A. Expected to cross react with JMJD2C (>90% similarity), JMJD2B (>85% similarity) due to high homology to these JMJD2 paralogs considering the antigen used. Observed detection of purified recombinant human JMJD2D at 50 ng sμggesting only ~10% cross reactivity with recombinant JMJD2D, however bands were detected at 60 kDa from cell samples.
Tag Line:Polyclonal antibody to detect JMJD2A (a lysine specific demethylase ) in human samples
Formulation:100 µl of antibody in TBS, pH 7.4, containing 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide.
Purification: Antigen affinity purified
Application:Western blot
Positive Control:Western blot: DLD1 cell lysate
Application And Usages:Western blot: 1:200 dilution. Not recommended for FACS and IHC based on probable cross reactivity with other JMJD containing proteins.
Handling:The antibody solution should be gently mixed before use.
Additional Information
Size: |
100 μl |
Antibody Target: |
JMJD2A |
Category: |
Epigenetics |
Lot Number: |
80508 |
Host: |
Rabbit |
Antibody Type: |
Polyclonal |
Isotype: |
Rabbit IgG |
Accession Number: |
O75164 |
Gene Id: |
9682 |
Concentration: |
N/A |
Appearance: |
Colorless liquid |
Form: |
Liquid |
Storage Condition: |
-20 °C |
Shipping Condition: |
gel pack |
Shelf Life: |
12 months |
Country of Animal Origin: |
China |
Country of Manufacture: |
USA |
Usage: |
For Research Use Only! Not to be used in humans. |
Western Blot Verified: |
Verified |
Immunocytochemistry Verified: |
Not Verified |
Immunofluorescence Verified: |
Not Verified |
Immunoprecipitation Verified: |
Not Verified |
ELISA Verified: |
Not Verified |
ChIP Verified: |
Not Verified |
Flow Cytometry Verified: |
Not Verified |