IL-33 Antibody

IL-33 Antibodyis available at Gentaur for Next week Delivery.

Description: Human IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities.

Immunogen Sequence:E.coli derived Recombinant Human IL-33

Target Alternative Name:NF-HEV

Species Reactivities:Human

Tag Line:Polyclonal antibody to detect IL-33 in human samples

Formulation:A sterile filtered antibody solution in PBS, pH 7.2.

Purification: Affinity chromatography

Application:Western blot, ELISA, Neutralization, Immunohistochemistry

Positive Control:ELISA: Recombinant hIL-33 (Cat # 4193) IHC: Human breast malignant ductal adenocarcinoma

Application And Usages:1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-33 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/ml), a concentration of 0.2-0.4 μg/ml of this antibody is required.
4) Immunohistochemistry: 0.125 μg/ml-0.25 μg/ml with an overnight incubation at 4°C

Handling:The antibody solution should be gently mixed before use.