IL-17A Antibody

IL-17A Antibodyis available at Gentaur for Next week Delivery.

Description: The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136 amino acid chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells.

Immunogen Sequence:E.coli derived Recombinant Human IL-17A

Target Alternative Name:CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte associated antigen 8

Species Reactivities:Human

Tag Line:Polyclonal antibody to detect IL-17A in human samples

Formulation:A sterile filtered antibody solution in PBS, pH 7.2.

Purification: Affinity chromatography

Application:Western blot, ELISA, Neutralization, Immunohistochemistry

Positive Control:ELISA: Recombinant hIL-17A (Cat # 4176) IHC: Human breast invasive ductal carcinoma tissue section

Application And Usages:1) WB: Use 0.1-0.2 µg/ml. The detection limit for recombinant human IL-17A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
2) ELISA: Use 0.5 - 2.0 μg/ml (100 μl/well antibody solution)
3) Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml), a concentration of 0.9-1.3 μg/ml of this antibody is required.
4) Immunohistochemistry: 0.25 µg/ml with an overnight incubation at 4°C.

Handling:The antibody solution should be gently mixed before use.