Description
His-Tag Antibody (Clone 11E12)is available at Gentaur for Next week Delivery.
Description: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
Immunogen Sequence:6X-HIS-KLH
Target Alternative Name:His-Tag
Species Reactivities:All
Tag Line:Monoclonal Antibody to detect His-taaged proteins
Formulation:1 mg/ml in 0.15 M PBS
Purification: Protein A chromatography from in vitro production
Application:WB, ELISA
Positive Control:Western blot: His-Tagged protein
Application And Usages:ELISA, WB
Handling:The antibody solution should be gently mixed before use.
Additional Information
Size: |
100 μl |
Antibody Target: |
His-Tag |
Category: |
His-Tag |
Lot Number: |
9C316714 |
Host: |
Mouse |
Antibody Type: |
Monoclonal |
Isotype: |
Mouse IgG1 κ |
Accession Number: |
N/A |
Gene Id: |
N/A |
Concentration: |
1 mg/ml |
Appearance: |
Colorless liquid |
Form: |
Liquid |
Storage Condition: |
-20 °C |
Shipping Condition: |
gel pack |
Shelf Life: |
12 months |
Country of Animal Origin: |
USA |
Country of Manufacture: |
USA |
Usage: |
For Research Use Only! Not to be used in humans. |
Western Blot Verified: |
Verified |
Immunocytochemistry Verified: |
Not Verified |
Immunofluorescence Verified: |
Not Verified |
Immunoprecipitation Verified: |
Not Verified |
ELISA Verified: |
Not Verified |
ChIP Verified: |
Not Verified |
Flow Cytometry Verified: |
Not Verified |