His-Tag Antibody (Clone 11E12)

His-Tag Antibody (Clone 11E12)is available at Gentaur for Next week Delivery.

Description: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

Immunogen Sequence:6X-HIS-KLH

Target Alternative Name:His-Tag

Species Reactivities:All

Tag Line:Monoclonal Antibody to detect His-taaged proteins

Formulation:1 mg/ml in 0.15 M PBS

Purification: Protein A chromatography from in vitro production

Application:WB, ELISA

Positive Control:Western blot: His-Tagged protein

Application And Usages:ELISA, WB

Handling:The antibody solution should be gently mixed before use.