H3K9me1 Antibody

H3K9me1 Antibodyis available at Gentaur for Next week Delivery.

Description: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K9 is associated with gene repression and heterochromatin formation, although higher levels of H3K9me1 have been found in some more active promoters.

Immunogen Sequence:KLH-conjugated synthetic peptide of Histone H3 containing monomethylated lysine 9.

Target Alternative Name:Histone H3

Species Reactivities:Human.

Tag Line:Polyclonal Antibody to detect H3K9me1 in human samples.

Formulation:In PBS with 0.05% (W/V) sodium azide.

Purification: Whole antiserum from rabbit

Application:Western blot, IF, ELISA, ChIP, Dot blot.

Positive Control:IF: HeLa cells, WB: HeLa cells, ELISA: Antigen, ChIP: Human osteosarcoma cells, Dot blot: Histone peptides

Application And Usages:IF: 1:200, WB: 1:1000, ELISA: 1:2000 – 1:3000, Dot Blot: 1:200,000, ChIP: 10 µl/ChIP.

Handling:The antibody solution should be gently mixed before use.