Flag-Tag Monoclonal Antibody

Flag-Tag Monoclonal Antibodyis available at Gentaur for Next week Delivery.

Description: DYKDDDDK-Tag (Flag-Tag) antibody detects over-expressed proteins containing the D epitope tag fused to either the amino- or carboxy-terminus of targeted proteins. Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The DYKDDDK epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.

Immunogen Sequence:/

Target Alternative Name:FLAG, DYKDDDDK

Species Reactivities:/

Tag Line:Monoclonal Antibody to detect Flag-taaged proteins

Formulation:100 ug (1 mg/ml) of antibody in 0.01M Tris-HCl, pH 8.0, 0.15M NaCl, and 0.02% sodium azide.

Purification: Affinity purified mouse IgG using protein G

Application:Western blot, Immunoprecipitation

Positive Control:N/A

Application And Usages:Western blot: 1-5 µg/ml Immunoprecipitation: 5μg antibody for 0.5 mg total cell lysates.

Handling:The antibody solution should be gently mixed before use.