DNA Methyltransferase 3a (Clone 64B814.1) Antibody

DNA Methyltransferase 3a (Clone 64B814.1) Antibodyis available at Gentaur for Next week Delivery.

Description: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The DNMT3 family members, DNMT3a and DNMT3b, are strongly expressed in embryonic stem (ES) cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of DNMT3b gene occur in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Immunogen Sequence:Recombinant mouse His-tagged DNMT3a expressed in bacteria

Target Alternative Name:DNMT3, DNMT3A

Species Reactivities:Human, mouse

Tag Line:Monoclonal antibody to detect DNMT3a in Human and Mouse samples

Formulation:50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.

Purification: Protein G purified

Application:Western blot, Immunofluorescence, Immunocytochemistry

Positive Control:Western blot: Transfected 293 cell lysate, untransfected 293 cell lysate

Application And Usages:Western blot: 1-3 µg/ml, IF/ICC: 5 µg/ml. However, the optimal conditions should be determined individually.

Handling:The antibody solution should be gently mixed before use.