DNA Methyltransferase 1 (Clone 60B1220.1) Antibody

DNA Methyltransferase 1 (Clone 60B1220.1) Antibodyis available at Gentaur for Next week Delivery.

Description: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. DNMT1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites sμggesting a link between DNA methylation, histone deacetylase, and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

Immunogen Sequence:Synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human DNMT1

Target Alternative Name:DNMT1

Species Reactivities:Human, mouse, zebrafish

Tag Line:Monoclonal antibody to detect DNMT1 in Human, Mouse and Zebrafish samples

Formulation:50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.

Purification: Antigen affinity purified

Application:Western blot, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation

Positive Control:N/A

Application And Usages:Western blot: 2-4 µg/ml. IHC (parffin-embedded sections), ChIP, and IP: 1-2 µg/ml. However, the optimal conditions should be determined individually.

Handling:The antibody solution should be gently mixed before use.