CD73/NT5E Antibody (CT)

CD73/NT5E Antibody (CT)is available at Gentaur for Next week Delivery.

Description: CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.

Immunogen Sequence:This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E).

Target Alternative Name:NT5E; NT5; NTE; 5'-nucleotidase; Ecto-5'-nucleotidase

Species Reactivities:Human, Mouse.

Tag Line:Polyclonal Antibody to detect CD73 in human and mouse samples.

Formulation:In PBS with 0.09% (W/V) sodium azide.

Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Application:Western blot, IHC.

Positive Control:Western blot: mouse brain tissue lysate, NT5E 293 ceel lysate. IHC: human placenta tissue.

Application And Usages:WB: 1:1000, IHC: 1:10 – 1:50.

Handling:The antibody solution should be gently mixed before use.