CD45 FITC Monoclonal Antibody (Clone HI30)

CD45 FITC Monoclonal Antibody (Clone HI30)is available at Gentaur for Next week Delivery.

Description: CD45, a transmembrane multifunctional glycoprotein, is a member of Type I receptor-linked PTPase family and is expressed as multiple isoforms due to alternative splicing. Expression of these isoforms is highly regulated and shift in this expression determines T-cell activation. CD45RB consists of exon B and is predominantly expressed in naïve T-cells secreting IL-2. Its expression is low in primed/memory T cells, cells that express Th2 cytokines such as IL-4 and IL-10 and population of T-cells with regulatory function. Immunotherapy with CD45RB antibody is being widely studied in transplantation and vaccination. CD45 antibodies are commonly used to identify tumors of lymphoid origin. The HI130 monoclonal antibody specifically reacts with the 180 kDa, 190 kDa, 205 kDa, and 220 kDa isoforms of the human leukocyte common antigen (LCA) CD45. It is expressed on lymphocytes, granulocytes, monocytes, thymocytes, and eosinophils, but not on mature erythrocytes, platelets, mature erythroid cells of bone marrow, and non-hematopoietic tissues. CD45 is essential for T cell activation and the tyrosine phosphatase activity of its intracellular region is integral for signal transduction.

Immunogen Sequence:Human CD45

Target Alternative Name:CD45

Species Reactivities:Human

Tag Line:Mouse monoclonal FITC conjugated antibody to detect CD45 in human samples.

Formulation:Phosphate-buffered aqueous solution pH 7.2, ≤0.09% Sodium azide, may contain carrier protein/stabilizer.

Purification: Affinity chromatography.

Application:Flow cytometry

Positive Control:FACS: Human peripheral blood lymphocytes

Application And Usages:Flow (Cell Surface): 5 µl/1x10^6 cells, Volume per test: 5 µl (0.25 µg).

Handling:The antibody solution should be gently mixed before use.