BMF

BMFis available at Gentaur for Next week Delivery.

Description: Bmf (for Bcl-2-modifying factor) is a novel BH3-only protein recently identified in human and mouse. The BH3 domain in Bmf is required for both binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptosis program.

Immunogen Sequence:Synthetic peptide surrounding amino acid 9 of human BMF

Target Alternative Name:BMF, FLJ00065, Bcl-2-modifying factor

Species Reactivities:Human, mouse, rat

Tag Line:/

Formulation:30 µg (0.5 mg/ml) affinity purified rabbit anti-BMF polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Application:Western blot, Immunohistochemistry

Positive Control:Western Blot: Jurkat cell lysate and Rat kidney tissue lysate. IHC: Kidney tissue

Application And Usages:Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~28 kDa BMF in sample from human, mouse and rat origins. Reactivity to other species has not been tested.

Handling:The antibody solution should be gently mixed before use.