Apaf-1 Antibody

Apaf-1 Antibodyis available at Gentaur for Next week Delivery.

Description: During apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.

Immunogen Sequence:Synthetic peptide surrounding amino acid 1161 of human Apaf-1

Target Alternative Name:APAF1, CED4, DKFZp781B1145, KIAA0413

Species Reactivities:Human, mouse, rat, bovine, rabbit, monkey, horse

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Formulation:100 µg (0.2 mg/ml) immunoaffinity purified, rabbit anti-Apaf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.05% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application:Western blot

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Application And Usages:Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.

Handling:The antibody solution should be gently mixed before use.