Anti-phospho-Akt (Ser473) Rabbit Monoclonal Antibody

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SKU:
A1119-50
€285
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Description

Anti-phospho-Akt (Ser473) Rabbit Monoclonal Antibodyis available at Gentaur for Next week Delivery.

Description: The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Immunogen Sequence:A phospho-peptide corresponding to human Phospho-Akt (Ser473)

Target Alternative Name:RAC serine/threonine-protein kinase; DRAC-PK; Dakt1; DAkt; Protein kinase B; PKB

Species Reactivities:Human

Tag Line:Rabbit monoclonal antibody to detect ACTA2 in human and mouse samples.

Formulation:In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Purification: Affinity purified using protein A

Application:IHC, WB

Positive Control:WB: 293 cells, IHC: breast cancer tissue section

Application And Usages:WB: 1:1000 -1:2000 dilution; IHC: 1:200 - 1:500 dilution.

Handling:The antibody solution should be gently mixed before use.

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Additional Information

Size:
50 µl
Antibody Target:
Phospho-Akt
Category:
Akt
Host:
Rabbit
Antibody Type:
Monoclonal
Isotype:
Rabbit IgG
Accession Number:
P31749
Gene Id:
207
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20 °C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
USA
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans.
Western Blot Verified:
Verified
Immunocytochemistry Verified:
Verified
Immunofluorescence Verified:
Not Verified
Immunoprecipitation Verified:
Not Verified
FACS Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Dot Blot Verified:
Not Verified
Flow Cytometry Verified:
Not Verified
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