Anti- His-Tag Rabbit Monoclonal Antibody

Anti- His-Tag Rabbit Monoclonal Antibodyis available at Gentaur for Next week Delivery.

Description: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.

Immunogen Sequence:Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Target Alternative Name:His-Tag

Species Reactivities:N/A

Tag Line:Rabbit monoclonal antibody to detect His-Tag.

Formulation:In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Purification: Affinity purified using protein A

Application:IP, WB, ICC, FC, IHC

Positive Control:WB: 293T cell lysate; IP: 293T cells; IHC: HepG2 cells; ICC: 293T cells

Application And Usages:Western Blot: 0.1 ug/mL – 1 ug/mL;Immunoprecipitation: 0.5 ug/mL – 2 ug/mL;Immunocytochemistry 0.5 ug/mL – 2 ug/mL;Flow Cytometry: 0.5 ug/mL – 2 ug/mL;Immunohistochemistry; 0.1 ug/mL-1 ug/mL.

Handling:The antibody solution should be gently mixed before use.