AKT1 (T450) Antibody (CT)

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SKU:
6743-100
€270
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Description

AKT1 (T450) Antibody (CT)is available at Gentaur for Next week Delivery.

Description: The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Immunogen Sequence:This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-457 amino acids from the C-terminal region of human AKT1.

Target Alternative Name:PKB, RAC, Protein kinase B, Protein kinase B alpha, Short PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase.

Species Reactivities:Human and mouse. Predicted cross reactivity with Rat and Bovine samples.

Tag Line:Polyclonal Antibody to detect AKT1 in human, mouse, rat, and bovine samples.

Formulation:100 µl of antibody in PBS with 0.09% (W/V) sodium azide

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Application:Western blot, IHC, FACS, IF.

Positive Control:Western blot: MCF-7 cell lysate and mouse cerebellum tissue lysate, IHC: human breast carcinoma tissue, FACS: MCF-7 cells, IF: MCF-7 cells.

Application And Usages:Western blot: ~1:1000, IHC: ~1:10-1:50, FACS: ~1:10-1:50, IF: ~1:10-1:50.

Handling:The antibody solution should be gently mixed before use.

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Additional Information

Size:
100 μl
Antibody Target:
AKT1
Category:
AKT1
Lot Number:
9E056743
Host:
Rabbit
Antibody Type:
Polyclonal
Isotype:
Rabbit IgG
Accession Number:
P31749
Gene Id:
207
Concentration:
N/A
Appearance:
Colorless liquid
Form:
Liquid
Storage Condition:
-20 °C
Shipping Condition:
gel pack
Shelf Life:
12 months
Country of Animal Origin:
USA
Country of Manufacture:
USA
Usage:
For Research Use Only! Not to be used in humans.
Western Blot Verified:
Verified
Immunocytochemistry Verified:
Verified
Immunofluorescence Verified:
Verified
Immunoprecipitation Verified:
Not Verified
ELISA Verified:
Not Verified
ChIP Verified:
Not Verified
Flow Cytometry Verified:
Verified
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