AKT1 Antibody (NT)

AKT1 Antibody (NT)is available at Gentaur for Next week Delivery.

Description: The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-∫ tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

Immunogen Sequence:This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the N-terminal region of human AKT1.

Target Alternative Name:PKB, RAC, Protein kinase B, Protein kinase B alpha, Short PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase.

Species Reactivities:Human. Predicted cross reactivity with mouse and Bovine samples.

Tag Line:Polyclonal Antibody to detect AKT1 in human, mouse, and bovine samples.

Formulation:100 µl of antibody in PBS with 0.09% (W/V) sodium azide

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Application:Western blot, IHC, FACS, IF.

Positive Control:Western blot: HeLa and 293 cell lysates, IHC: Human breast carcinoma, FACS: MDA-MB435 cells, IF: MDA-MB435 cells.

Application And Usages:Western blot: ~1:1000, IHC: ~1:50-1:100, FACS: ~1:10-1:50, IF: ~1:10-1:50.

Handling:The antibody solution should be gently mixed before use.