Leptin, rat recombinant

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SKU:
4368-5
€185
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Description

Leptin, rat recombinant is available at Gentaur for Next week delivery.

Adipose-derived hormone which regulates energy intake & energy expenditure

Biomolecule/Target: Leptin

Alternates names: OB Protein, Obesity Protein, OBS, Obesity factor.

Synonyms: OB Protein, Obesity Protein, OBS, Obesity factor.

Background Information: Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.

Reconstitution Instructions: Reconstitute in H₂O to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for three months. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

NCBI Gene Symbol: LEP

Gene ID: 25608

NCBI Accession: P50596

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Additional Information

Size:
5 mg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Rat
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
≥95%
Assay 2:
HPLC
Endotoxin Level:
<0.1 ng/μg
Activity (Specifications/test method):
N/A
Biological activity:
The ED₅₀ determined by the leptin-dependent stimulation of human OB-R transfected murine BaF3 indicator cells is 0.35-0.06 ng/ml.
Results:
0.35-0.06 ng/ml
Molecular Weight:
16.2 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized protein
Handling:
Centrifuge the vial prior to opening.
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