GPI, human recombinant

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SKU:
7822-100
€185
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Description

GPI, human recombinant is available at Gentaur for Next week delivery.

A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate

Biomolecule/Target: GPI

Alternates names: Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36.

Synonyms: Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36.

Background Information: Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Reconstitution Instructions: N/A

NCBI Gene Symbol: GPI

Gene ID: 2821

NCBI Accession: P06744

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Additional Information

Size:
100 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. coli.
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
< 1.0 EU per 1 μg of protein (determined by LAL method)
Activity (Specifications/test method):
N/A
Biological activity:
Specific activity is > 90 units/mg, and one unit will convert 1.0 µmole D-fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 25ºC.
Results:
Specific activity is > 90 units/mg
Molecular Weight:
65.3 kDa (578 aa, 1-558 aa + His Tag)
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
1 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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