Description
GPI, human recombinant is available at Gentaur for Next week delivery.
A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate
Biomolecule/Target: GPI
Alternates names: Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36.
Synonyms: Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36.
Background Information: Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: GPI
Gene ID: 2821
NCBI Accession: P06744
Additional Information
Size: |
100 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 1.0 EU per 1 μg of protein (determined by LAL method) |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Specific activity is > 90 units/mg, and one unit will convert 1.0 µmole D-fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 25ºC. |
Results: |
Specific activity is > 90 units/mg |
Molecular Weight: |
65.3 kDa (578 aa, 1-558 aa + His Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
1 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |