EZCut™ TEV Protease, Recombinant

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SKU:
7847-1000
€185
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Description

EZCut™ TEV Protease, Recombinant is available at Gentaur for Next week delivery.

≥95% Pure active, highly site specific and stable recombinant TEV Protease

Biomolecule/Target: TEV Protease

Alternates names: Nuclear inclusion protein A, NIa protein

Synonyms: Nuclear inclusion protein A, NIa protein

Background Information: BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4°C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut™ TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

Reconstitution Instructions: N/A

NCBI Gene Symbol: N/A

Gene ID: N/A

NCBI Accession: N/A

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Additional Information

Size:
1 mg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Rabbit
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.
Biological activity:
The EZCut™ TEV Protease has an activity of ≥10,000 units/mg.
Results:
≥10,000 units/mg
Molecular Weight:
28.6 kDa (2038–2279 aa + C-terminal poly-his tag).
Storage Temperature:
-80°C
Shelf Life:
12 months
Concentration:
1 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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