Description
EZCut™ TEV Protease, Recombinant is available at Gentaur for Next week delivery.
≥95% Pure active, highly site specific and stable recombinant TEV Protease
Biomolecule/Target: TEV Protease
Alternates names: Nuclear inclusion protein A, NIa protein
Synonyms: Nuclear inclusion protein A, NIa protein
Background Information: BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It contains an enhanced form of a catalytic fragment of the NIa protein of Tobacco etch virus (TEV). TEV Protease is a restriction grade protease that has robust activity at 4°C with high specificity and great stability. The optimal temperature for cleavage with this enzyme is 34°C. The protease can be used for the removal of affinity tags from fusion proteins. It contains a C-terminal His tag and can be easily removed after cleavage reactions by passing the reaction through a Ni-chelating resin. BioVision’s EZCut™ TEV Protease is an improved version of TEV protease that is highly site-specific, highly active, and significantly more stable than native TEV protease, resulting in enhanced long-term activity.
Reconstitution Instructions: N/A
NCBI Gene Symbol: N/A
Gene ID: N/A
NCBI Accession: N/A
Additional Information
Size: |
1 mg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Rabbit |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
The EZCut™ TEV Protease has an activity of ≥10,000 units/mg. |
Biological activity: |
The EZCut™ TEV Protease has an activity of ≥10,000 units/mg. |
Results: |
≥10,000 units/mg |
Molecular Weight: |
28.6 kDa (2038–2279 aa + C-terminal poly-his tag). |
Storage Temperature: |
-80°C |
Shelf Life: |
12 months |
Concentration: |
1 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |