Caspase-3, rat recombinant

Caspase-3, rat recombinant is available at Gentaur for Next week delivery.

A cysteine-aspartic acid protease involved in apoptosis

Biomolecule/Target: Caspase-3

Alternates names: Caspase-3, Short name=CASP-3, Apopain, Cysteine protease CPP32, Short name=CPP-32, IRP, LICE, Protein Yama, SREBP cleavage activity 1, Short name=SCA-1

Synonyms: Caspase 3

Background Information: Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).

Reconstitution Instructions: Reconstitute to 1 unit per µl in PBS containing 15% glycerol.

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