Ebola virus-like particles (EBOV VLP)

Ebola virus-like particles (EBOV VLP) is available at Gentaur for Next Week Delivery.

Product Type: protein

Host: N/A

Clonality: N/A

Applications: WB

Description: Virus-like Particles (VLP’s) expressing recombinant Ebola virus (EBOV)
glycoprotein (GP), nucleoprotein (NP), and matrix protein (VP40). These VLP’s are produced in Sf9
insect cells through infection with a recombinant baculovirus.

Storage: 2-3 weeks at -20°C, -80°C long term

Size: 1 mg of protein supplied in PBS (supplemented with arginine and glutamic acid) at a concentration of 2.403 mg/mL.

Endotoxin: 2.30 Endotoxin Units/mg

Relevance: Since these VLP’s mimic Ebola virus but do not contain genetic material, thus are not
infectious and make an ideal candidate as a vaccine and as a tool to enhance filovirus research.

Western Blot Detection

Western Blot was used to confirm the presence of
EBOV-specific GP, NP and VP40 in the VLP. VLP
sample was heat-denatured under reducing
condition and loaded at 1 µg/lane.
(A) GP was detected as a broadly-diffused band 60-
110 kDa using 50 ng/mL of anti-EBOV GP rabbit
Pab (cat# 0301-015)
(B) NP was detected as a single sharp band at ~90
kDa using 100 ng/mL of anti-EBOV NP rabbit Pab
(cat# 0301-012)
(C) VP40 was detected as a single sharp band at
~40 kDa using 50 ng/mL of anti-EBOV VP40 rabbit
Pab (cat# 0301-010)