Rat Prothrombin Fragment 1+2 (F1+2) ELISA Kit (CLIA)

Rat Prothrombin Fragment 1+2 (F1+2) ELISA Kit (CLIA) is available at Gentaur for next week delivery.

Specificity: This assay has high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2).

Cross Activity: This assay has high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2).

Test principle: Average the duplicate readings for each standard, control, and samples and subtract the average zero standard relative light unit (RLU). Create a standard curve on log-log graph paper, with F1+2 concentration on the y-axis and the RLU value on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, such as curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 100 µL Substrate Solution. Incubate 10 minutes at 37°C; 8. Read RLU value immediately.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Restrictions: For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.

Quality Systems: The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Quality Assurance: Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

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Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.