Rat PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit

Rat PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit is available at Gentaur for next week delivery.

Specificity:

Cross Activity: No significant cross-reactivity or interference was observed.

Test principle: This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PACAP. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PACAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PACAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PACAP. The concentration of Rat PACAP in samples can be calculated by comparing the OD of the samples to the standard curve.

Assay procedure summary:

Stability:

Restrictions: For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.

Quality Systems: The kit is manufactured at ISO 9001 certified facilities.

Quality Assurance:

Research Area: Neuroscience

Target Synonyms: PACAP

Precision: