Rat PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit

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SKU:
EKE62367
Availability:
Next Week Delivery
€659
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Description

Rat PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit is available at Gentaur for next week delivery.

Specificity:

Cross Activity: No significant cross-reactivity or interference was observed.

Test principle: This ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat PACAP-38. During the reaction, Rat PACAP-38 in the sample or standard competes with a fixed amount of Rat PACAP-38 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PACAP-38. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Rat PACAP-38 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay procedure summary:

Stability:

Restrictions: For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.

Quality Systems: The kit is manufactured at ISO 9001 certified facilities.

Quality Assurance:

Research Area: Neuroscience

Target Synonyms: PACAP-38

Precision:

 

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Additional Information

Size:
96-Well Plate
Species Reactivity:
Rat
Sample type:
Serum, Plasma, Biological Fluids
Detection range:
7.81-500 pg/mL
Sensitivity:
4.69 pg/mL
Detection Method:
Colorimetric
Assay Time:
2.5 hours
Application:
ELISA
Assay Type:
Competitive
Shipping Condition:
Ice packs
Storage:
Short term: 4°C; Long term: see manual.
Precaution of Use:
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date:
6 months
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