Biomatik Elisa
Mini Samples Lipopolysaccharide (LPS) ELISA Kit
- SKU:
- EKU11214
- Availability:
- Next Week Delivery
Description
Mini Samples Lipopolysaccharide (LPS) ELISA Kit is available at Gentaur for next week delivery.
Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Lipopolysaccharide (LPS).
Cross Activity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Lipopolysaccharide (LPS).
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Lipopolysaccharide (LPS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Lipopolysaccharide (LPS) and unlabeled Mini Samples Lipopolysaccharide (LPS) (Standards or samples) with the pre-coated antibody specific to Mini Samples Lipopolysaccharide (LPS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Lipopolysaccharide (LPS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Lipopolysaccharide (LPS) in the sample.
Assay procedure summary:
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Restrictions: For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems: The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Quality Assurance: Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.
Research Area:
Target Synonyms: LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Lipopolysaccharide (LPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Lipopolysaccharide (LPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.
Additional Information
Size: |
96-Well Plate |
Target Name: |
Lipopolysaccharide |
Species Reactivity: |
All species |
Sample type: |
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Detection range: |
12.35-1,000 ng/mL |
Sensitivity: |
4.53 ng/mL |
Detection Method: |
Colorimetric |
Assay Time: |
3 hours |
Application: |
ELISA |
Assay Type: |
Double-antibody Sandwich |
Shipping Condition: |
Ice packs |
Storage: |
Short term: 4°C; Long term: see manual. |
Precaution of Use: |
The Stop Solution is acidic. Do not allow to contact skin or eyes. |
Expiry Date: |
8 months |
UniProt ID: |
n/a |